Lab Report Eugenol

grounding Eugenol is a natur all in ally occurring chemical that has aesculapian applications such as its use as a natural dental anesthetic. It ass be extracted from cloves by utilise the manner of steam clean distillation. The extracted compound will whence be separated from the water using methyl chloride, which will therefore be evaporated using a rotary evaporator going completely the essential oil, Eugenol. IR spectrographic analysis will be used to countersink the structure of the extracted compound. Results and Discussion Four samples of Eugenol were unite to be analyzed for the final yield.A dismount per centum recovery, as indicated by control board 1 could be due to the situation that much(prenominal) of the weight of the cloves was not in truth eugenol. Cloves Used Eugenol Recovered Percent retrieval 10 g 7. 304 (7. 304/40) x light speed% = 18. 26% evade 1 Calculations of the percent recovery of Eugenol. Figure 1 The structure of Eugenol. The IR spectroscopy run on the sample gave results as shown in Table 2. The OH blossom out accounts for the hydroxyl group on the resonant ring as shown in Figure 1.The C-H peaks are from the mexthoxy group on the aromatic ring. The C=C-H are a result of the hydrogens bonded to the carbons of the aromatic ring. The peak from the C=O thin is not from the compound, but may be a result of product contamination. Absorbance contrive Intensity Group-motion 3512. 21 Broad anemic H2O or OH arrival 3072. 52 Sharp Medium C=C-H stretch 2948. 85 Broad Strong C-H stretch 2845. 80 Sharp Medium C-H stretch 1767. 18 Sharp Weak C=O stretch Table 2 IR Table of sample taken.Experimental line of descent of Eugenol began by taking 10 grams of cloves and placing them in a 250mL round tail flaskful. This flask was then attached to a steam distillation apparatus and heated to the stewing point of about 100C. The steam was con mutedd and collected in a gradatory cylinder. This collected material consi sted of water and the eugenol contained in the cloves. 100mL of the product was collected and placed into a separatory move. The eugenol now had to be removed from the water by adding 15mL of CH2Cl2 and shaken.The floors were allowed to separate. The CH2Cl2 layer was more dense than water and sank to the bottom of the funnel. The bottom layer was collected and another 15mL of dichloromethane was added to the funnel to repeat the process. This was repeated yet once more to obtain a final substance of 45mL of dichloromethane with eugenol dissolved in it. The 45mL of product was moved to a 100mL Erlenmyer flask. The next note was to dry the mixture with 0. 5g of CaSO4 to remove some(prenominal) excess water in the mixture.The flask was swirled to allow for the CaSO4 to collect any water. The product was then allowed to rest and was put finished a filter to remove the CaSO4. at once filtered, four different samples were collected in a single 500mL round bottom flask. This product was then placed onto a rotary evaporator to evaporate away all of the dichloromethane and leave behind only eugenol. This was achieved because the boiling point of the dichloromethane was much lower than that of eugenol. The final product was then analyzed by IR spectroscopy.